Table 1.
Summary of retinal degeneration observed in transgenic X. laevis
| Type of transgenic tadpole | GFP expression | Number examined | Proportion with degeneration (%) |
|---|---|---|---|
| GFP-rab8WT primary transgenic | + | 10 | 20 |
| GFP-rab8Q67L primary transgenic | + | 11 | 55 |
| GFP-rab8T22N primary transgenic | + | 12 | 100 |
| GFP-rab8WT F1 (founder A) | + | 8 | 0 |
| GFP-rab8Q67L F1 (founder B) | + | 6 | 100 |
| GFP-rab8Q67L F1 (founder B) | − | 4 | 0 |
| GFP-rab8Q67L F1 (founder C) | + | 4 | 0 |
| GFP-rab8T22N F1 (founder D) | + | 11 | 100 |
| GFP-rab8T22N F1 (founder D) | − | 4 | 0 |
| GFP-rab8T22N F1 (founder E) | + | 6 | 100 |
| Offspring of nontransgenics | − | 8 | 0 |
Summary of results from H&E histology of primary transgenic animals and F1 offspring. All animals were sacrificed at 14 dpf, except for GFP-rab8T22N primary transgenics, where two animals were sacrificed at 9 dpf, six animals at 12 dpf, and four at 14 dpf. Retinal degeneration was observed only in animals expressing fusion protein (GFP expression +) and not in nonexpressing offspring of transgenic animals (GFP expression −), or offspring of nontransgenic animals.