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Primer sequences for q-PCR.
| Target gene | Sequences (5′-3′) |
|---|---|
| Collagen Ι | F, AGTGGTTTGGATGGTGCCAA; R, GCACCATCATTTCCACGAGC |
| RUNX2 | F, AGTGGTTTGGATGGTGCCAA; R, CACTTCTAGGTCTGACGACG |
| OCN | F, TCTATGACCTGCAGAGGGCT; R, ATAGCTCGTCACAAGCAGGG |
| OSX | F, TCTCAAGCACCAATGGACTCCT; R, GGTAGTCATTTGCATAGCCAGA |
| GAPDH | F, CAGGGCTGCCTTCTCTTGTG; R, GATGGTGATGGGTTTCCCGT |
F, forward; R, reverse; OCN, osteocalcin; Runx2, runt-related transcription factor 2; GAPDH, D-glyceraldehyde-3-phosphate; OSX, osterix.
