Figure 4.

Effect of IL-6 suppression on colony formation and cell invasion. ((a) and (b)) Soft-agar assay was performed in the cells with stably expressing control vector or the shIL-6 and treated with or without 100 mg/kg gemcitabine. Data presented were from three independent experiments with duplicates in each experiment. ((c) and (d)) Invasiveness of the cells with stably expressing control vector or the shIL-6, treated with or without gemcitabine. Cellular invasive capability was determined with a modification of the Boyden chamber invasion assay as described in the Materials and Methods and representative images were presented in (c). The percentage of invasive cells from three separate experiments (mean ± SD) was presented as bar graphs (d). ^∗P < 0.05.