Skip to main content
. 2018 Mar 6;2018:3195025. doi: 10.1155/2018/3195025

Figure 5.

Figure 5

Effect of IL-6 suppression on STAT3 signaling and apoptosis-associated proteins. (a) Effect on the expression of caspase-3, caspase-9, IL-6, STAT3, JAK2, and Bcl2 mRNA. Expression of the indicated mRNA was quantified by real time RT-PCR in the cells after 48 hours of shIL-6 transfection followed by treatment with or without gemcitabine. P < 0.05 compared with the nontreated control (NC). ((b) and (c)) Protein levels of STAT3, p-STAT3, JNK, p-JNK, Bcl-2, Bax, caspase-3, and caspase-9. Levels of the indicated proteins were semiquantitatively determined by immunoblot in the cells 48 hours after shIL-6 transfection followed by treatment with or without gemcitabine. Representative image data (b) as well as an average of three separate experiments (c) were presented. P < 0.05 compared with shIL-6 + gemcitabine.