Figure 2.

GFP-TAM67 inhibits HT1080 colony formation. GFP, TAM67, and GFP-TAM67 were cloned into the bicistronic vector pIRESpuro. HT1080 cells were then transfected with these constructs in duplicate. (A) One set of transfections was plated in triplicate in DMEM, 10% fetal calf serum, and 0.5 μg/ml puromycin. Colonies were counted after 14 d. The figure is representative of three independent experiments. (B) Lysates were made from the other set of transfections and probed with anti-c-jun antibodies; lane 1, GFP; lane 2, TAM67; lane 3, GFP-TAM67.