Figure 3.

Ponasterone A induces GFP-TAM67. The iGT1a clone was treated with either vehicle (A) or 10 μM ponasterone A (B) for 24 h. Cells were fixed, stained with Alexafluor⁵⁶⁸-conjugated phalloidin and imaged by laser scanning confocal microscopy. (C) Immunoblot analysis of lysates from clone iGT1a treated with (+) and without (−) ponasterone A probed with antibodies to c-jun. A short and a long exposure of this blot is presented. (D) Immunoprecipitation of lysates isolated under denaturing or nondenaturing conditions from [³⁵S]methionine-labeled, uninduced or induced iGT1a cells with anti-GFP or control immunoglobulin G.