Figure 4.

Ponasterone A induction of GFP-TAM67 inhibits AP-1 reporter gene activity. Control iC1 cells and iGT1a cells were treated with 10 μM ponasterone A or vehicle for 24 h and then transiently transfected with constructs driving a heat-stable SEAP reporter gene. Alkaline phosphatase activity from heat–inactivated conditioned media normalized to β-galactosidase activity is presented. The promoter constructs used were: tal, an enhancerless minimal promoter; ctrl, a full SV40 early promoter; pAP-1, a minimal promoter with four tandem repeats of the consensus AP-1 site, TGA(G/C)TCA.