Figure 2.

PTPN22 regulates T‐cell dependent IL‐17 responses induced by curdlan stimulated BMDCs in vivo. Wild type (WT) and Ptpn22 ^−/− derived bone marrow derived dendritic cells (BMDCs) were pulsed overnight with OVA_323‐339 in the presence or absence of curdlan. BMDCs were harvested and injected into the left footpad of OT‐II mice. Seven days post immunization draining popliteal lymph nodes were isolated and the number of cells within the draining (A) lymph nodes determined by Trypan blue counting. Total draining (B) lymph node T‐cells were stimulated with immobilized anti‐CD3 for 48 h and cell‐free supernatant assayed for IL‐17, IFNγ and TNFα by immunoassay. Data are representative of three independent experiments with four mice per group per experiment, each data point representing an individual OT‐II mouse lymph node. Bars represent the mean ± S.D. NS = not significant, **p < 0.01, ***p < 0.001 by two‐way ANOVA, applying Sidak's multiple comparisons test.