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. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 3.

Figure 3

Ptpn22 regulates BMDC IL‐1β secretion in response to curdlan. (A) WT and Ptpn22−/ − derived bone marrow derived dendritic cells (BMDCs) were stimulated for 24 h in the presence or absence of curdlan. Cell‐free supernatants were assessed for IL‐1β, IL‐6, IL‐12/23p40, and TNFα by immunoassay. Data are pooled from 8 to 12 independent experiments with one sample per group per experiment, and represent mean ± S.D. NS = not significant, **p < 0.01, ***p < 0.001 applying two‐way ANOVA, with Sidak's multiple comparisons test. (B) WT BMDCs were incubated in the presence or absence of α‐dectin‐1 or IgG2a isotype control antibody for 30 min prior to stimulation for 24 h in the presence or absence of curdlan. Cell‐free supernatants were assessed for IL‐1β by immunoassay. Data are pooled from five independent experiments with one sample per group per experiment, and represent mean + S.E.M. NS = not significant, *p < 0.05, **p < 0.01 applying one‐way ANOVA, with Holm‐Sidak's multiple comparisons test.