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. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 4.

Figure 4

Curdlan stimulated Ptpn22 −/− BMDCs induce enhanced IL‐17 responses in an IL‐1β dependent manner. WT and Ptpn22 −/− derived bone marrow derived dendritic cells (BMDCs) were pulsed overnight with OVA323‐339 in the presence or absence of curdlan. BMDCs were harvested and injected into the left footpad of OT‐II mice in the presence or absence IL‐1RA. 7 days post immunization the draining (left) popliteal lymph nodes were isolated. Total draining lymph node T‐cells were stimulated with immobilised anti‐CD3 for 48 h and cell‐free supernatants assayed for IL‐17, IFNγ and TNFα by immunoassay. Data are representative of two independent experiments with 3–5 mice per group per experiment, each point representing an individual OT‐II mouse lymph node. Data represent mean ± S.D. NS = not significant, *p < 0.05, **p < 0.01, ***p < 0.001 by two‐way ANOVA, applying Sidak's multiple comparisons test.