Skip to main content
. 2017 Oct 20;48(2):306–315. doi: 10.1002/eji.201747092

Figure 5.

Figure 5

Ptpn22 regulates dectin‐1 dependent Syk and Erk activation in BMDCs. (A–C) WT and Ptpn22 −/− BMDCs were stimulated with heat killed C. albicans (HKCA) for the indicated time points. Whole cell lysates were resolved by SDS‐PAGE and immunoblotted using specific antibodies to pSyk (Tyr525/526) or Syk, and pErk1/2 (p42/44) or Erk. Quantification using ImageJ software of HKCA induced band intensity measurements pooled from five independent experiments are shown for pSyk (B) and pErk (C), relative to Syk and Erk, respectively. Phosphorylated Syk protein values were normalized to total protein and the fold change to 0 min calculated. Data are mean + S.E.M. from five independent experiments with one sample per group per experiment. *p < 0.05 applying two‐way ANOVA, with Sidak's multiple comparisons test.