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. 2018 Mar 13;9:435. doi: 10.3389/fimmu.2018.00435

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Copyright © 2018 Serve, Sturm, Schimunek, Störmann, Heftrig, Teuben, Oppermann, Horst, Pfeifer, Simon, Kalbas, Pape, Hildebrand, Marzi and Relja.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of CD25⁺CD127⁻ lymphocytes in CD4⁺ lymphocytes. (A,F) Gating strategy for CD4⁺ lymphocytes in human and porcine peripheral blood before trauma. (B–E) Gating strategies for human CD25⁺CD127⁻ lymphocytes. (G–J) Gating strategies for human CD25⁺CD127⁻ lymphocytes. (K) Relative counts of CD25⁺CD127⁻ lymphocytes in comparison in percent. Means and SEM are shown. HV: healthy volunteers; AT: immediately after trauma; D1: 24 h after trauma; D3: 72 h after trauma; polytrauma patients: n = 20, HV: n = 10, animals: n = 10; *significantly different (p < 0.05) to HV or ctrl, respectively.