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Working procedure for the serum SEP detection. According to the workflow for the enrichment and identification of low abundance mouse serum proteins (A), two rounds of mouse serum proteins were separated by SDS-PAGE and stained by Colloidal blue. B The first round, seven WT mice. C The second round, four WT mice and four ob/ob mice. The proteins below 14 kDa (the proteins between two red lines in every lane) were sliced for mass spectrometric analysis
