Fig. 1.

Longitudinal levels of serum alanine aminotransferase (ALT) in the anti-PD-1-treated hepatitis patient. Day 0 represents the day ALT levels exceeded Grade 3 levels. Grade 3 is defined as > 5× upper normal limit (ULN) and Grade 4 > 10× ULN. Therapeutic interventions used to control hepatitis are indicated above the graph. ATG anti-thymocyte globulin. Days when blood samples were processed for PBMCs and cryopreserved are indicated by asterisks, annotated by assay. CyTOF: mass cytometric analysis. Rhodamine: rhodamine 123 efflux fluorescence based analysis