Fig. 1.

Generation of PI4KIIα transgenic (TG) mice. A Workflow to generate PI4KIIα TG BALB/c mice. B Islets and acinus were isolated from the mouse pancreas, and PI4KIIα expression was measured by Western blot. C The pancreases of WT and PI4KIIα TG littermates were sectioned at 10 µm using a cryostat. Protein expression in pancreas sections was determined using antibodies against insulin and PI4KIIα. Images were obtained using a laser confocal fluorescence microscope. Scale bar, 40 μm. D Islets were isolated from the pancreas of 25-week-old male PI4KIIα TG mice (line 17) and age-matched WT littermates (N = 5 for each line), and PI4P content was measured using a PI(4)P Mass Strip Kit. The data are presented as the mean ± SD. All the experiments except A were performed three times in triplicate. *p < 0.05