Table 2.
Yeast strain | Cells with normal actin morphology
(%)
|
|
---|---|---|
26°C | 38°C | |
Wild type | >95 | >95 |
stt4ts | >90 | <10 |
sac1ts | >90 | >90 |
stt4ts/sac1ts | >90 | >60 |
Percentages are based on >200 cells observed for each strain at each condition. Wild type (SEY6210), stt4ts (AAY102), sac1Δ (MFY62) carrying URA3 CEN6 sac1-23, and double mutant stt4ts/sac1Δ (MFY55) carrying URA3 CEN6 sac1-23 cells at 26°C and 38°C are described. Comparison of stt4ts and stt4ts/sac1ts cells is highlighted at 38°C.