Figure 2.

Fat-specific knockout of Rheb promotes lipolysis in mice. A: The mRNA levels of lipolytic and lipogenic genes in sWAT of Rheb^fKO (KO) and control mice (4 months old; n = 6/group) were determined by quantitative real-time PCR. B: Western blot analyses of FAS, ACC, P-HSL, HSL, and ATGL protein levels in sWAT of Rheb^fKO and control mice. P, phosphorylated. Glycerol release (C) and FFA release (D) from primary subcutaneous white adipocytes isolated from Rheb^fKO and control (Ctrl) mice (n = 3/group) treated with or without 10 μmol/L isoproterenol (Iso) for 6 h at 37°C. Data are mean ± SEM. *P < 0.05; **P < 0.01.