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. 2017 Jan 23;66(4):948–959. doi: 10.2337/db16-0636

Figure 4.

Figure 4

Syn-2 deletion enables formation of more profusion SM/SNARE complexes. A: SM/SNARE protein profile of adrenal glands from WT and Syn-2–KO mice. B: Syn-2 co-IP of cognate SM/SNARE proteins in WT adrenal gland lysates. Representative of two experiments. Co-IP of Syn-1A in C or Syn-3 in D pulled down SM/trans-SNARE complexes from WT versus Syn-2–KO mice adrenal glands. Representative of three independent experiments with samples performed in duplicate; respective densitometry analyses shown in bottom panels. Values from WT adrenals were used as control (100%); all other values were normalized to control. E: Syn-2 co-IP of cognate SNARE/SM proteins in control INS-1 cells, treated as indicated; analysis in Supplementary Fig. 4D. F: Syn-2 siRNA knockdown in INS-1 cells compared with scrambled siRNA. Representative of three experiments; analysis in Supplementary Fig. 4A and B (protein levels) and Supplementary Fig. 4C (insulin secretion). G and H: Co-IP of Syn-1A in G or Syn-3 in H pulled down cognate SNARE/SM proteins in Syn-2 siRNA versus control scrambled nonsense siRNA-transduced INS-1 cells, treated as indicated. Representative of three independent experiments with samples performed in duplicate; analysis in Supplementary Fig. 4E and F. *P < 0.05; **P < 0.01.