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. 2016 Aug 23;65(11):3396–3409. doi: 10.2337/db16-0136

Figure 4.

Figure 4

FH deficiency reduces TG synthesis in white adipocytes, allows normal stimulation of lipolysis, and impairs temperature maintenance. A: TG synthesis assessed in isolated perigonadal white adipocytes as described in research design and methods (n = 6–7). B: Correlation between ATP depletion and reduced TG synthesis (perigonadal adipocytes, 5-month-old mice, n = 6). C: Plasma NEFA levels at 5 and 48 h of fasting (5-month-old males, n = 9). D: Changes in NEFA levels following injection of saline or β3-adrenergic–specific agonist CL 316,243. Mice were fasted for 5 h and then injected intraperitoneally with saline or CL 316,243 in saline. Tail-vein blood samples were collected before and 15 min after injection for NEFA assay (5-month-old males, n = 12). E: Body temperature at 4°C after 4 h of fasting (4-month-old males, n = 12). F: Body temperatures at 21°C after 5 and 48 h of fasting (5-month-old males, n = 9). G: Thermogenesis-related transcripts in BAT (5-month-old males, n = 8). H: UCP1 protein expression in BAT measured by Western blot in control and AFHKO mice (5-month-old males, 30 μg protein/lane). *P < 0.05; **P < 0.01; ***P < 0.001. Ant, antimycin A; CL, CL 316,243; NS, not significant; Olig, oligomycin; Rot, rotenone; Tm, temperature.