Table 1.

Estimating mtDNA copy number using randomly selected autosomal DNA fragments, individual chromosomes and whole genome

# of fragments	Fragment length (bp)	Total length	Time	R2	R2 Range in 20 simulations
1	100	0.1K	4.6 s	0.154	[0.095, 0.243]
100	1	0.1K	6.6 s	0.932	[0.900, 0.945]
1	1000	1K	4.6 s	0.311	[0.183, 0.480]
1000	1	1K	22 s	0.989	[0.975, 0.993]
300	1000	300K	12 s	0.991	[0.984, 0.996]
10 000	100	1M	96 s	0.999	[0.998, 0.999]
3000	1000	3M	59 s	0.998	[0.997, 0.999]
30 000	1000	30M	386 s	0.999	[0.999, 0.999]
Chromosome 20		63M	6 min	0.981	N/A
Chromosome 1		249M	23 min	0.999	N/A
Whole Genome		∼3B	3 h	1.0	N/A

The different options are ordered by the total length of autosomal DNA being considered in the analysis from the smallest to the largest. The option in bold is selected as the default option in fastMitoCalc.