Table 3. Effect of OLE loaded NLC on hematological, inflammatory mediators and cyclooxygenase enzymes.
| Treatment | Hematological parameters | Inflammatory mediators | COX Inhibition (%) | |||||
|---|---|---|---|---|---|---|---|---|
| RBC (million/cu.mm) |
WBC (cells/cu.mm) |
ESR (mm/h) |
Hb (gm%) |
TNF-α (ng/mL) |
IL-1 (ng/mL) |
COX-1 | COX-2 | |
|
Group-I: OLE-FREE-NLC-Gel (Normal control) |
7.11 ± 0.83 | 6.6 ± 0.65 | 2.4 ± 0.31 | 13.4 ± 1.50 | 0.13 ±0.05 | 0.38 ± 0.07 | --- | --- |
| Group-I I: CFA (Toxic control) | 4.13 ± 0.06# | 9.81± 1.2# | 3.2 ± 0.52# | 9.8 ± 0.81# | 0.45 ±0.10# | 0.0.98±0.06# | --- | --- |
| Group-III: CFA+OLE-NLC -Gel | 6.74 ± 0.08* | 7.12 ± 0.05** | 2.7 ± 0.20 ** | 12.5± 1,2** | 0.16 ±0.06 | 0.44± 0.05** | 48± 5.76 | 60±10.42 |
|
Group-IV: CFA + Marketed Gel (1.16% diclofenac) |
6.40 ± 0.04** | 7.5 ± 0.03 | 2.6 ± 0.41** | 11.9± 0,87** | 0.14 ±0.03** | 0.42 ± 0.08* | 57 ± 8.4 | 50 ± 8.87 |
Values expressed as mean ± standard error of mean.
#p<0.01 as compared to control group
*p<0.05
**p<0.01 as compared to toxic control.
The comparisons were made by ANOVA followed by Dunnett’s test