Figure 2.

tubg1 alanine-scanning mutants that show conditional growth. A 10-fold dilution series containing 10⁵–10² cells of (A) dominant cold-sensitive (cs⁻) or TUBG1, (B) recessive cs⁻ mutants or TUBG1 in the background of a γ-tubulin null mutant (C) recessive cs⁻ mutants that were sensitive to the microtubule-depolymerizing drug TBZ or TUBG1 in the background of a γ-tubulin null mutant were plated on appropriate selective media with or without 10 μg/ml TBZ. The plates were then incubated at a permissive temperature of 30°C or a restrictive temperature of 18°C for 7 d. Panels shown in C were all incubated at the permissive temperature of 30°C.