Figure 5.

Cold-sensitive tubg1 mutants undergo abnormal mitosis. Dominant cs⁻ cells (A and B), recessive cs⁻ cells (C and D), and wild-type cells expressing TUBG1 were grown to mid-log phase at 30°C and then shifted to 18°C (A and C) or left at 30°C for 10 h (B and D). Cells were then fixed and microtubules were stained with the anti-α-tubulin, monoclonal antibody TAT-1 (1:50) followed by Cy3-conjugated goat anti-mouse secondary antibody. DNA was stained with 1 μg/ml DAPI. Arrows indicate mitotic cells.