Table 1.
Growth phenotypes of alanine-scanning mutants of TUBG1 in S. pombe
| Mutant | Amino Acid Change | with gtb1+ | without gtb1+ |
|---|---|---|---|
| tubg1-1 | R3A, E4A | wt | wt |
| tubg1-2 | E20A, K23A | wt | Recessive lethal |
| tubg1-3 | E38A, E39A, E43A | wt | wt |
| tubg1-4 | D46A, R47A, K48A, D49A | wt | cs− |
| tubg1-5 | D56A, D57A, E58A | cs− | wt |
| tubg1-6 | D68A, E70A, P71A, R72A | wt | cs− |
| tubg1-7 | K84A, L85A, Y86A, N87A, P88A, E89A | wt | cs− |
| tubg1-8 | D120A, D123A, R124A, E125A, D127A | wt | Recessive lethal |
| tubg1-9 | S129A, D130A, S131A, E133A | wt | Recessive lethal |
| tubg1-10 | E155A, R156A, L157A, D159A, R160A | wt | cs− |
| tubg1-11 | K163A, K164A, L165V | wt | Recessive lethal |
| tubg1-12 | D159A, R160A, K163A, K164A | wt | Recessive lethal |
| tubg1-13 | D176A, E177A, S179A, D180A | cs− | Recessive lethal |
| tubg1-14 | K193A, R194A, L195A | wt | cs− |
| tubg1-15 | R286A, K287A, T288A | wt | cs− |
| tubg1-16 | D292A, R295A, R296A, L297A | wt | wt |
| tubg1-17 | R309A, D310A, R311A | wt | wt |
| tubg1-18 | R339A, R341A, E342A, R343A, K344A | cs− | Recessive lethal |
| tubg1-19 | R362A, K363A, S364A | wt | wt |
| tubg1-20 | E389A, R390A, T391A, R393A | wt | wt |
| tubg1-21 | D396A, K397A, R399A, K400A, R401A, E402A | wt | wt |
| tubg1-22 | E406A, R409A, K410A, E411A, D412A | cs− | wt |
| tubg1-23 | K415A, D416A, N417A | wt | Recessive lethal |
| tubg1-24 | D419A, E420A, D422A | wt | Recessive lethal |
| tubg1-25 | R425A, E426A, I427A | wt | wt |
| tubg1-26 | D433A, E434A, Y435A | cs− | Recessive lethal |
| tubg1-27 | R440, P441A, D442A | cs− | Recessive lethal |
wt, wild type.