Table 1.
Oligonucleotides used in this study
Oligo No. | Used to constructa | Strand | Sequence |
---|---|---|---|
1. | Upper | 5′-CCGGTGATAATAGGATCCTCTAGA-3′ | |
b(5)-R | |||
2. | Lower | 5′-AGCTTCTAGAGGATCCTATTATCA-3′ | |
3. | Upper | 5′-CCGGCGGAGCAGAATGAACGGAGGAACAGAAGGACCAAACTTCTACGTACCATTCAG- CAACAAAACAGTAGACAGCAGATGAA-3′ | |
b(5)-RRNglyc | |||
4. | Lower | 5′-AGCTTTCATCTGCTGTCTACTGTTTTGTTGCTGAATGGTACGTAGAAGTTTGGTCCTTCT- GTTCCTCCGTTCATTCTGCTCCG-3′ | |
5. | Upper | 5′-TCGAAGGTGGAGGAGGTTCAGGAGGAGGTGGATCTGGAGGTGGAGGTGCGGATCCT-3′ | |
Synthetic linker | |||
6. | Lower | 5′-TCGAAGGATCCGCCACCTCCAGATCCACCTCCTCCTGAACCTCCTCCACCT-3′ | |
7. | Upper | 5′-CTAGACTGCAGTCATTAACG-3′ | |
GFP-RR | |||
8. | Lower | 5′-CCGGCGTTAATGACTGCAGT-3′ | |
9. | Upper | 5′-CTAGACTGCAGCTATCATTA-3′ | |
GFP-R | |||
10. | Lower | 5′-CCGGTAATGATAGCTGCAGT-3′ | |
11. | Upper | 5′-CTAGACTGCAGTCATTACGTGTACATCAGCGCTACAACCAGAGCAGAGATCGCAGGG- ATAACCCAGTTAGTCCACCAA-3′ | |
GFP-Thr | |||
12. | Lower | 5′-TCGAGTTGGTGGACTAACTGGGTTATCCCTGCGATCTCTGCTCTGGTTGTAGCGCTGAT- GTACACGTAATGACTGCA-3′ |
See Figure 1 for a schematic representation of the constructs.