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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Steroids. 2017 Sep 20;142:2–5. doi: 10.1016/j.steroids.2017.09.004

Figure 2.

Figure 2

Denaturation-purified Dex-BSA (100 nM) retained its effect on GR nuclear translocation. A. N42 cells expressing GR-GFP were treated with purified Dex-BSA and imaged at 0, 5, and 20 min. Lower panels show magnifications of the boxed regions in the upper panels. B. Time course of GR-GFP translocation to the nucleus, measured by fluorescence intensity in a nuclear region of interest. The kinetics of GR nuclear translocation induced by the unpurified (Dex-BSA) and the purified Dex-BSA were comparable to each other, but were both slower than the free Dex-induced nuclear translocation of GR (Dex).