Fig. 5.

Role of IRAK1 and STAT6 in CCL24 induction by IL-13 and IL-33 in murine BMDMs. a CCL24 protein levels in culture medium of IRAK1 KO and WT mouse BMDMs stimulated with IL-13 and IL-33 for 48 h. n = 9 replicates from 3 independent experiments. b BMDMs from Tollip KO and WT mice were incubated with IL-13 and IL-33 for 24 h: a representative Western blot of phosphorylated IRAK1 (pIRAK1) and total IRAK1 proteins (left); densitometric analysis of IRAK1 activation, as determined by the ratio of pIRAK1 to total IRAK1 (right). n = 9 replicates from 3 independent experiments. c Representative Western blot of phosphorylated STAT6 (pSTAT6) and STAT6 in Tollip KO and WT mouse BMDMs stimulated with IL-13 and IL-33 for 60 min (left); densitometric analysis of STAT6 activation, as determined by the ratio of pSTAT6 to total STAT6 (right). n = 3 replicates from 2 independent experiments. Data are expressed as means ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001.