Figure 3.

Cdc42 deficiency increases disease severity of colitis. (A) Body weight loss of Cdc42^+/+Lck^Cre and Cdc42^fl/flLck^Cre mice treated with DSS in drinking water or normal water only. The data are expressed as percentage of initial body weight. (B) Representative flow cytogram of IL-17- and IFN-γ-producing CD4⁺ cells in the lamina propria of DSS-treated Cdc42^+/+Lck^Cre and Cdc42^fl/flLck^Cre mice. Numbers in dot plots indicate percentages of IL-17- and IFN-γ-producing CD4⁺ cells (left). Average percentages of IL-17- and IFN-γ-producing CD4⁺ cells are shown in bar graph (right). (C) Body weight loss of RAG1^−/− mice transferred with naïve CD4⁺ T cells from Cdc42^+/+Lck^Cre and Cdc42^fl/flLck^Cre mice. (D) Average percentages of IL-17⁺, IFN-γ⁺ and IL-17⁺IFNγ⁺ cells in the spleen, mLN and lamina propria of RAG1^−/− mice in (C). n = 5. Error bars indicate SD. *p < 0.05, **p < 0.01.