Figure 4.

Cdc42 deficiency leads to an impaired iTreg differentiation and stability in vitro and in vivo. (A) Representative flow cytogram of CD4⁺ T cells from Cdc42^+/+Lck^Cre and Cdc42^fl/flLck^Cre mice cultured under iTreg polarizing condition and stained for IL-17 and Foxp3. Numbers in dot plots indicate percentages of Foxp3⁺, Foxp3⁺IL-17⁺ and Foxp⁻IL-17⁺ cells (left). Average percentages of Foxp3⁺, Foxp3⁺IL-17⁺ and Foxp⁻IL-17⁺ cells are shown in bar graph (right). (B) Representative flow cytogram of cells from (A) stained for IL-17 and IFN-γ. Numbers besides dot plots indicate percentages of IL-17⁺IFN-γ⁺ and IFN-γ⁺ cells in corresponding quadrant (left). Average percentages of IL-17⁺IFN-γ⁺ and IFN-γ⁺ cells are shown in bar graph (right). (C) Representative flow cytogram of CD4⁺Foxp3⁺ cells in the lamina propria of Cdc42^+/+Lck^Cre and Cdc42^fl/flLck^Cre mice treated with or without DSS. Numbers indicate percentages of CD4⁺Foxp3⁺ cells. (D) Average percentages of CD4⁺Foxp3⁺ cells in the spleen, mLN and lamina propria of RAG1^−/− mice transferred with naïve CD4⁺ T cells from Cdc42^+/+Lck^Cre or Cdc42^fl/flLck^Cre mice. n ≥ 5. Data are representative of three independent experiments. Error bars indicate SD. *p < 0.05, **p < 0.01.