(A) Liver-to-body weight (LBW) ratio, serum aminotransferases [alanine transaminase (ALT) and aspartate transaminase (AST)] and alkaline phosphatase (ALP) from mice fed chow, methionine- and choline-deficient (MCD), or Western diets. (B) Hematoxylin and eosin (H&E) staining of representative liver sections from mice (left) and NASH patients with mild or severe fibrosis (right). (C) Oil red O staining of representative liver sections from mice (left) and liver lipid levels (right). (D) qRT-PCR analysis of liver genes encoding lipid metabolic enzymes. The results were normalized to chow diet-fed mice and graphed as mean ± SEM. *p < 0.05 and **p < 0.005, control versus experimental diet; #p < 0.05 and ##p < 0.005, MCD versus Western diet. Reprinted with permission from Machado et al.46 per Creative Commons Attribution (CC-BY) license.