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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Immunol. 2018 Feb 23;200(7):2327–2340. doi: 10.4049/jimmunol.1701391

Figure 2. The characterization of DNA binding by variably citrullinated forms of LL-37.

Figure 2

(A) The efficiency of DNA binding by native and differentially citrullinated forms of LL-37 (LL-377, LL-377,29, LL-377,29,34, and fully citrullinated LL-37) estimated with surface plasmon resonance analysis. Response units (RU) were used to describe the amounts of coupled DNA. (B) The efficiency of modified LL-37 to protect DNA against degradation represented as the percentage of DNA bound to the peptide after 60 min of DNase treatment. Statistical significance was evaluated by one-way ANOVA, followed by Tukey’s multiple comparisons post-test (****P<0.0001). (C) Citrullination of CRAMP affects the peptide binding to bacterial DNA visualised by retardation assay.