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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Immunol. 2018 Feb 14;200(7):2291–2303. doi: 10.4049/jimmunol.1600924

Figure 9. Overexpression of HspA1A is sufficient to inhibit TLR4-MyD88 dependent cytokine TNFα but not TLR4-MyD88 independent chemokine RANTES.

Figure 9

RAW macrophages were transiently transfected with pCMV5-HspA1A using 1:1, 1:2 and 1:3 ratios of 2 μg DNA to lipofectamine. 24 hours after transfection, macrophages were stimulated with 100 ng/ml LPS for 6 hours and culture supernatants were assayed for secreted (A) TNFα and (B) RANTES by ELISA. Graph depicts mean ± SD (n=4) and statistical analysis was performed by one way ANOVA with Dunnett’s test for multiple comparisons. (ns p>0.05, * p<0.05, ** p<0.01, *** p<0.001, ****p=0.0001)