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. 2001 Aug;12(8):2519–2533. doi: 10.1091/mbc.12.8.2519

Figure 8.

Figure 8

Cnm67p acts as a spacer between intermediate layer 2 and the outer plaque. (A) Visual comparison of the variations in the IL2-to-outer plaque distance of the coiled-coil mutants. Two independent series of SPBs are shown in a–c and d–f. The SPBs were aligned with their IL2 (bottom line) and the middle of the outer plaques were marked (top three lines). a and d represent wild-type SPBs, b and e are cnm67 Δcoils SPBs, and c and f are SPBs of cnm67 2coils cells. The scale subdivisions correspond to 20 nm units. (B) Statistical evaluation of the IL2-to-outer plaque distances. The columns represent the average distance between the centers of the IL2 and outer plaque, measured on SPB density profiles along the spindle axis; the error bars mark the standard deviation ς (see text). (C) Thickness of CNM67, cnm67 Δcoils, and cnm67 2coils mutants' outer plaques were measured on SPB density plots; ς is represented by the error bars.