Table 3.
Plasmids used in this study
| Plasmid | Markers/vector | Insert | Source or reference |
|---|---|---|---|
| pRS414 | Amp, ori, CEN, ARS, TRP1 | Sikorski and Hieter, 1989 | |
| pUC19 | Amp, ori | Yanisch-Perron et al., 1985 | |
| pYGFP1 | Amp, ori | yEGFP1(F64L, S65T) | Cormack et al., 1997 |
| pFA6a-3HA-kanMX6 | pFA6a | 3HA-TADH-kanMX6 | Brachat et al., 1998 |
| pFA6a-GFP(S65T)-HIS3MX6 | pFA6a | GFP(S65T)-TADH-HIS3MX6 | Wach et al., 1997 |
| pYM3 | pFA6a | 6HA-klTRP1 | Knop et al., 1999 |
| pFS040 | pFA6a | (GA)3-yEGFP1-6H | This study |
| pFS042 | pFA6a | (GA)3-3HA-6H | This study |
| pFS043 | pFA6a | (GA)10-yEGFP1-6H | This study |
| pFS045 | pFA6a | (GA)10-3HA-6H | This study |
| cosmid14-6 | Amp, ori | 19.054kb region of chromosome 14 left arm | Philippsen collection |
| pFS011 | pRS414 | CNM67 | This study |
| pFS019 | pRS414 | CNM67(SmaI) | This study |
| pFS022 | pRS414 | CNM67∷(GA)3-yEGFP1-6H | This study |
| pFS024 | pRS414 | CNM67∷(GA)3-3HA-6H | This study |
| pFS048 | pRS414 | CNM67∷(GA)10-3HA-6H | This study |
| pFS025 | pRS414 | cnm67Δcoil1(SmaI) | This study |
| pFS026 | pRS414 | cnm67Δcoil2(SmaI) | This study |
| pFS027 | pRS414 | cnm67Δcoil3(SmaI) | This study |
| pFS028 | pRS414 | cnm67ΔC(SmaI) | This study |
| pFS029 | pRS414 | cnm67ΔN(SmaI) | This study |
| pFS030 | pRS414 | cnm67Δcoil1∷(GA)3-yEGFP1-6H | This study |
| pFS031 | pRS414 | cnm67Δcoil2∷(GA)3-yEGFP1-6H | This study |
| pFS032 | pRS414 | cnm67Δcoil3∷(GA)3-yEGFP1-6H | This study |
| pFS033 | pRS414 | cnm67ΔC∷(GA)3-yEGFP1-6H | This study |
| pFS034 | pRS414 | cnm67ΔN∷(GA)3-yEGFP1-6H | This study |
| pFS035 | pRS414 | cnm67Δcoil1∷(GA)3-3HA-6H | This study |
| pFS036 | pRS414 | cnm67Δcoil2∷(GA)3-3HA-6H | This study |
| pFS037 | pRS414 | cnm67Δcoil3∷(GA)3-3HA-6H | This study |
| pFS038 | pRS414 | cnm67ΔC∷(GA)3-3HA-6H | This study |
| pFS039 | pRS414 | cnm67ΔN∷(GA)3-3HA-6H | This study |
| pFS049 | pRS414 | cnm67_Cterm(SmaI) | This study |
| pFS089 | pRS414 | cnm67_Cterm∷(GA)10-yEGFP1-6H | This study |
| pFS090 | pRS414 | cnm67_Nterm∷(GA)10-3HA-6H | This study |
| pFS093 | pRS414 | cnm67Δcoil1-3(SmaI) | This study |
| pFS094 | pRS414 | cnm67Δcoil1-3∷(GA)10-yEGFP1-6H | This study |
| pFS095 | pRS414 | cnm67Δcoil1-3∷(GA)10-3HA-6H | This study |
| pFSC63 | pUC19 | CNM67 (SmaI) | This study |
| pFS103 | pUC19 | cnm67_2coils | This study |
| pFS105 | pRS414 | cnm67_2coils∷(GA)10-yEGFP1-6H | This study |
| pFS106 | pRS414 | cnm67_2coils∷(GA)10-3HA-6H | This study |
| pFS092 | pRS414 | CNM67∷(GA)3-3HA-6H∷HIS3MX6 | This study |
| pFS118 | pRS414 | cnm67ΔC∷(GA)3-3HA-6H∷HIS3MX6 | This study |
| pFS119 | pRS414 | cnm67ΔN∷(GA)3-3HA-6H∷HIS3MX6 | This study |
| pFS131 | pRS414 | cnm67Δcoil1-3∷(GA)10-3HA-6H∷HIS3MX6 | This study |
| pFS133 | pRS414 | cnm67_2coils∷(GA)10-3HA-6H∷HIS3MX6 | This study |