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. 2018 Mar 20;8:4892. doi: 10.1038/s41598-018-23045-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Luciferase reporter assays using the promoter region of the LCE1C gene. (a) A schematic representation of the promoter region (~2000 bp) of LCE1C. Green box indicates the location of the luciferase gene. (b) Bar graph to indicate the promoter activity of #1–4 promoter regions (arbitrary units). Promoter activity of SV40 promoter was measured as a positive control. Promoter activities using no TAp63 DNA (NT) or vector alone (Vec) in the reaction mixture were also measured as negative controls. (c) Bar graph to examine the alteration of the promoter activity of LCE1C-promoter #1 by addition of Myc-TAp63-WT, Myc-TAp63-T46A/T281A (AA), or Myc-TAp63-T46D/T281D (DD) plasmids into the reaction mixture. Promoter activities using no TAp63 DNA (NT) or vector alone (Vec) in the reaction mixture were also measured as negative controls (NC). Addition of SV40 promoter was examined as a positive control (PC). N.S. indicates no significant difference. (d,e) Wb to confirm the exogenous expression of Myc-TAp63 in luciferase assays. α-tubulin was detected as a loading control. (f) A schematic representation of the action of the GAK_TAp63-T46/T281_LCE1C axis (i), which was destroyed in the Myc-TAp63-T46A/T281A (AA) expressing cells (ii).