Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 20;8:4900. doi: 10.1038/s41598-018-22618-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparison of DENV-3 disease in adult AG129 mice. (a) Kaplan-Meier survival curves of mice infected with 10^7.0 (n = 11) or 10^7.7 (n = 9) f.f.u. of C0360/94 or 10^7.0 (n = 2), 10^7.5 (n = 3), or 10^8.0 (n = 4) f.f.u. of D83-144 via the i.p. route. Mice were monitored for at least 4 weeks. (b) Percent weight lost during infection relative to animal weight prior to infection, each line represents the time course of a single animal. (c–g) Mice inoculated with 10^7.5 f.f.u. D83-144 or C0360/94 were sacrificed daily to determine viral loads during acute infection. (c) Blood was collected and serum samples were used for viremia measurement; results are from three independent experiments. Differences in daily viral titers between D83-144 and C0360/94 were determined by Mann-Whitney tests and asterisks denote significance; day 2 p = 0.0003, day 3 p = 0.0011, day 1 was not significant, p = 0.0545 (denoted by #). (d–g) Internal organ samples were homogenized, and virus was titrated; results are pooled from two separate experiments. (d) Liver viral titers were significantly different on day 1 (p = 0.0030) and day 2 (p = 0.0466). (e) Spleen viral titers were significant on day 1 (p = 0.0061) and day 2 (p = 0.0012). (f) Viral titers in the large intestine were significant on day 2 only (p = 0.0344) and trended significant on day 3, p = 0.0669 (denoted by #). Viral titers in the brain were low for both D83-144 and C0360/94, but were significantly different on day 2 (p = 0.0128). Serum titers are expressed as log₁₀ f.f.u./ml and organ titers as log₁₀ f.f.u./g of tissue. Symbols represent mean sample titers, error bars: SEM. L.O.D. as follow: serum: 10^2.0 f.f.u.; liver: 10^2.8 f.f.u.; spleen: 10^2.8 f.f.u.; large intestine: 10^3.0 f.f.u.; brain: 10^2.7 f.f.u.