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. 2018 Mar 20;8:4900. doi: 10.1038/s41598-018-22618-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.

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Blood profile of D83-144-infected mice. (a) Levels of platelets, percent hematocrit, leukocytes, and lymphocytes were determined from EDTA-anticoagulated blood from control (n = 10) or D83-144-infected mice on day 1 (n = 5), day 2 (n = 7), or day 3 (n = 7). Results are pooled from two separate experiments; lines represent the mean daily value, and comparisons between control and infected samples were determined with ANOVA with post-test. (b) Effects of D83-144 or C0360/94 infection on coagulation were determined on day 3 p.i. using the Vetscan PT/aPTT test (prothrombin time/activated partial thromboplastin time) on sodium citrate-anticoagulated blood from mock- (n = 4), D83-144- (n = 3) or C0360/94-infected (n = 4) mice.