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. 2018 Mar 20;8:4907. doi: 10.1038/s41598-018-23261-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isolated and combined HFD plus 3xtg background determine a clear-cut separation in microbiota composition. Beta-diversity PCA using weighted (a) and unweighted-UniFrac distances (b). RDA plot at OTU level between groups (c, colon; d, caecum). Each symbol represents a single sample shaped and coloured according to the respective group.