Fig. 2. moDC stimulation with B0213Δprn induced increased pro-inflammatory cytokine production compared to B0213 or B0213REprn stimulation.

MoDCs were stimulated with B0213, B0213Δprn or B0213REprn at an MOI of 10 or left unstimulated for 48 h. Using luminex, moDC production of a IL-6, b TNF-α, c IL-8, and d G-CSF in the supernatant and surface expression, using flow cytometry, of e CD80, f CD83, and g CD86 was determined. Experiments were performed using at least three donors *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001