Table 1.
Lethal exposure doses. Percent mortality of wild type embryos at 24 hpf and 48 hpf. N = 14–15 biological replicates.
| ControlΨ | Vehicle control* | 15 μM BPA | 50 μM BPA | 60 μM BPA | 70 μM BPA | 80 μM BPA | 90 μM BPA | 100 μM BPA | 200 μM BPA | |
|---|---|---|---|---|---|---|---|---|---|---|
| 24 hpf | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 100% |
| 48 hpf | 0% | 0% | 0% | 0% | 6.7% | 0% | 0% | 26.7% | 100% | NA |
Embryos were subject to chronic non-static exposure of treatment starting at 6 hpf in all groups.
ΨE3 media.
*1% DMSO dissolved in E3 media.
NA: this dose was 100% lethal to embryos at 24 hpf.
Abbreviations: BPA – Bisphenol A; hpf – hours post fertilization.