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. 2017 Dec 14;37(9):1175–1191. doi: 10.1038/s41388-017-0034-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — EV miR content controls β-catenin activation. a TECs that over-express miR-214-3p were subjected to SDS–PAGE to evaluate β-catenin content, normalized to β-actin. Untransfected TECs were used as internal controls (none) (n = 5) (**p < 0.01, none vs pre-miR-214-3p, unpaired t-test). b Cell extracts from TECs, left untreated or treated with EVs, anti-IL-3R-EVs or EVs enriched in miR-214-3p, were analyzed for β-catenin content, normalized to β-actin (n = 5) (**p < 0.01, none and EVs vs anti-IL-3R-EVs and pre-miR-214-3p-EVs, one-way ANOVA). c, d Cytoplasmic extracts from TECs depleted of miR-24-3p (antago-miR-24-3p) (c) and from TECs treated with antago-miR-24-3p-EVs (d) were analyzed by Western blot for APC and GSK3β content (**p < 0.01, none vs antago-miR-24-3p in (c), unpaired t-test, and **p < 0.01, EVs vs anti-IL-3R-EVs and antago-miR-24-3p-EVs in d, one-way ANOVA). e, f Cell extracts from antago-miR-24-3p TECs (e) and from TECs, treated as above (f), were analyzed for pβ-catenin and β-catenin content, normalized to β-actin (n = 4) (*p < 0.05, for pβ-catenin and **p < 0.01, for β-catenin, none vs antago-miR-24-3p in e, unpaired t-test, and **p < 0.01, none and EVs vs anti-IL-3R-EVs and antago-miR-24-3p-EVs in f, one-way ANOVA)