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. 2018 Mar 20;8:4878. doi: 10.1038/s41598-018-23165-0

Figure 5.

Figure 5

Flow cytometric analysis of renal granulocyte infiltration. (A) Gating strategy: Pre–gating on live cells using Fixable Viability Dye eFluor 660 and further gating on single cells. (B) Representative flow cytometry data of infiltrating Ly6G–positive cells (granulocytes) and F4/80–positive cells (macrophages) in sham and I/R–injured kidneys of WT and Trpv4 KO mice. (C) Quantification of infiltrating Ly6G–positive cells and (D) F4/80-positive cells. n = 4 for sham-operated WT and Trpv4 KO mice and n = 6 for I/R–injured WT and Trpv4 KO mice, two-way ANOVA, Sidak’s multiple comparisons test. In both cases PTreatment < 0.0001, and PGenotype and PInteraction are not significant.