Fig. 5.

Architecture of the NuA4 TEEAA-piccolo assembly. a Schematic view of subunit and modular organization of the NuA4 complex, highlighting the subunits present in the TEEAA-piccolo assembly. b Comparison of the cryo-EM structures of the TEEAA-piccolo and TEEAA assemblies in the front view. The density map is color coded by subunit assignment as panel a. The green dashed oval indicates the binding position of the piccolo module. c Three views of the TEEAA-piccolo assembly. The cryo-EM density is shown as a translucent surface and fitted with the ribbon diagram model of the piccolo crystal structure (PDB ID 5J9U). Arp4 is in yellow, actin in purple, Tra1 in orange, HSA domain of Eaf1 in red, Eaf5 in cyan, and the piccolo module in green. Each successive view is rotated as indicated. d Enlarged view of the piccolo assembled into the NuA4 complex. Epl1 is in dark-green, Esa1 in lime, Eaf6 in yellow-green, and Yng2 in light-green. Piccolo interacts with both Eaf1 and Tra1 subunits. The Tra1 HEAT 24R and 41R mainly responsible for piccolo binding are highlighted in red pipes