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. 2018 Mar 20;8:4901. doi: 10.1038/s41598-018-22043-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival of isolated intracellular T. cruzi amastigotes following exposure to either posaconazole or nifurtimox for 48 hours, followed by differentiation and growth in liquid medium. Amastigotes were isolated from infected 3T3 cells following treatment with either 1.00 µM of posaconazole or 12.0 µM of nifurtimox for 48 hours. Parasites were differentiated in LIT and grown for 5 weeks until in the log phase of growth before enumerating.