Table 1.
Primers used for RT-qPCR.
| Gene | Forward primers | Reverse primers |
|---|---|---|
| Rpl19 | gggcaggcatatgggcata | ggcggtcaatcttcttggatt |
| Tbp | ggggagctgtgatgtgaagt | ccaggaaataattctggctca |
| Abhd5 | atctttggagcccgatcct | cttctggctgatctgcatacac |
| Pnpla2 | tgaccatctgccttccaga | tgtaggtggcgcaagaca |
| Plin5 | Agctggagcccgtgtcta | gggcatccactgcattct |
| Lipe HSL | gcctggcaaaatctgagg | gcctccgtggatgtgaac |
| Lpl | tttgtgaaatgccatgacaag | cagatgctttcttctcttgtttgt |
| CerS1 | ggagctactgcgcttacctg | ccatgcctgacctccagt |
| Clock | ccagtcagttggtccatcatt | tggctcctaactgagctgaaa |
| Bmal1 | cgactcattgatgccaagac | tgcacttcattctacagaacagaga |
| Cidea | ttcaaggccgtgttaagga | cctttggtgctaggcttgg |
| Cpt1b | Ctcctttcctggctgaggt | gatctggaactgggggatct |
| Prdm16 | Cctaaggtgtgcccagca | caccttccgcttttctaccc |
| Dio2 | tggcatgccctgtaggtt | tgagaagtctggtacatcagcaa |
| Ucp1 | ggctctgcaggagtccgaagt | ggcgtgagtgcaagaacaaaa |
| Ucp2 | tttgggtttggttgtttttga | ggaggcagatgcaggtagat |
| Ucp3 | ctgcctgtccatcagttcaa | ccagtcctaacaattccctaaaga |
| AdRβ3 | cagccagccctgttgaag | ccttcatagccatcaaacctg |
Rpl19, ribosomal protein L19; Tbp, TATA-box binding protein; Abhd5, α/β hydrolase domain containing 5; Pnpla2, patatin-like phospholipase domain containing 2 (ATGL); Plin5, perilipin5; Lipe HSL, lipase hormone sensitive; Lpl, lipoprotein lipase; CerS1, ceramide synthase1; Clock, clock circadian regulator; Bmal1, brain and muscle Arnt-like protein-1; CIDEA, cell death-inducing DNA fragmentation factor alpha subunit-like effector A; Cpt1b, carnitine palmitoyltransferase 1B; PRDM16, PR/SET domain 16; Dio2, type 2 iodothyronine deiodinase; UCP, uncoupling protein; AdRβ3, β3-adrenergic receptor.