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. 2018 Mar 14;9:175. doi: 10.3389/fphys.2018.00175

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Copyright © 2018 Koyyada, Latchooman, Jonaitis, Ayoub, Corcoran and Casalotti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preference assay for naive or ethanol exposed flies. Preference indices were measured at 2 and 24 h in the same batch of flies. Columns represent three independent experiments, each consisting of three assay tubes containing 6–8 flies each. n = 9. Error bars are SEM. Statistical significance was measured by two-way ANOVA with Bonferroni multiple comparisons test. The effect of alcohol treatment was highly significant p < 0.0001 (horizontal line with^*). The effect of time was not significant p = 0.635.