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. 2018 Mar 14;8:71. doi: 10.3389/fcimb.2018.00071

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Copyright © 2018 Sundar, Islam, Braza, Silver, Le Breton and McIver.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of pathways for glucose uptake in MGAS5005. (A) Schematic of predicted PTS (green) and non-PTS (blue) pathways for glucose transport in GAS leading to glucose-6-phosphate. Indicated are a putative glucose-specific PTS EII system, a predicted non-PTS GlcU glucose permease and the NagC glucokinase. (B) Growth assays (%WT) at 12, 24, 36, and 48 h (as shown in grid) were performed on GAS mutants in the entire PTS (ΔptsI), a PTS EIICD locus (ΔmanMN) important for glucose utilization in other streptococci, the GlcU permease (ΔglcU) and the NagC glucokinase (ΔnagC) were grown in CDM + various % glucose as described in section Materials and Methods. Growth above WT (green), 2-fold below WT (yellow), < 2-fold below WT (red), and similar to WT (white) are indicated. (C) ΔOD₆₀₀ was calculated for the same mutants compared to MGAS5005 and outcomes indicated as described for (B). Red boxes indicated total yields statistically significantly below wildtype (p < 0.05), determined using a student's t-test.