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. 2018 Mar 18;11(3):401–407. doi: 10.18240/ijo.2018.03.09

Figure 1. HE staining of lens anterior capsule in manual group (A-D), FLACS1 group (E-H), and FLACS2 group (I-L).

Figure 1

The capsule cutting edge irregularity and roughness in two FLACS groups (E, I) and smooth edge in manual group (A). Lens epithelium cells showed a regular distribution and shape with even disperse chromatin in manual group (B) but irregularities of the cell configuration with partly swollen and destroyed nuclei were observed in two FLACS groups under 1000× magnified immersion objective (F, J). Lens epithelium cell dropped from anterior capsules severely after standard fixation, embedding and staining procedures and cell number significantly decreased in two FLACS groups (G, H, K, L) compared with the manual group (C, D). The lens anterior capsule edge in FLACS2 group was more irregular than that in FLACS1 group (E, I). The exfoliated cells in FLACS1 group had a distance of approximately 3-4 LECs rows, while only 1-2 LECs rows distance could be found in FLACS2 group (H, L).