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. 2018 Mar 21;38(2):BSR20171490. doi: 10.1042/BSR20171490

Figure 1. CRISPR/Cas9-mediated editing of SRY in rabbits.

Figure 1

(A) Schematic diagram of the two sgRNAs targeting the SRY loci. The yellow rectangle represents the HMG region of SRY. Two sgRNAs sequences, sgRNA1 and sgRNA2, are highlighted in green. Protospacer adjacent motif (PAM) sequences are in red. Primers F and R were used to detect mutations in the blastocysts and pups. (B) PCR and T7E1 cleavage assay for detecting mutations in organs of the SRY-mutant chimeric rabbits. M, D2000. (C) TIDE mutation detection assay for multiple organs in the SRY-mutant chimeric rabbits. Deletions are denoted as ‘−’ and numbers represent the percentage of the mutant type.