Figure S1.

Dual sgRNA-directed mutation of SRY in zygotes. (A) Mutation detection in blastocyst by PCR. M, marker; 1#–13# represent different male blastocysts used in this study. M DNA marker. (B) Mutation detection in blastocyst by T-cloning and Sanger sequencing. The WT sequence is shown at the top of the targeting sequence. sgRNA sequences are marked in green and the protospacer adjacent moti (PAM) sequences are in red with underline. WT: wild type; deletions ‘-’; insertion ‘+’.

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